|
What is a good way to test the production of
carbon dioxide in humans after different types of
exercise? What is the very best method to measure
that? My teacher wants me to use a better method
other than having the person blow into a cup of
water with a straw for 30 seconds immediately
after the exercise, then testing to see what the
pH of the water is because CO2 is
acidic. Thank you very much.
|
Question Date: 2007-01-23 | | Answer 1:
Actually the method you have devised for measuring
CO2 output is a clever one. The more
CO2 you add to water, the more the pH
will indeed fall. However your teacher makes a
valid point in that while this relationship is
true, it may be difficult to measure what you are
trying to measure in this manner, for two reasons.
First, it is very difficult to use an approach
like this in a way that is consistent. For
example, you can never control how hard a person
blows over the thirty second time period, and the
CO2 will also quickly escape from the
water and equilibrate into the air! Second, even
if you could control for those things you may be
trying to measure a very small change that could
be difficult to see in pH.
Unfortunately,
the best way to measure the CO2 content
that is being out gassed in an experiment like
this is probably with a professional device that
will likely be expensive (such as a gas
chromatography machine or the like). There are
certain to be several commercial kits for this
purpose-- for example, air pollution monitoring
kits-- or you may be able to borrow a device from
somebody who uses such a tool regularly (such as
the rebreather/analyzers used by exercise
physiologists, or a gas analyzer from a heating
and air conditioning professional). There are
also several chemical methods that you could try,
but these would most likely also be subject to the
same problems as your current method. | | Answer 2:
I'm guessing here, but I think your teacher is
afraid that you won't see much difference in the
pH of the water before and after exercise. You
have the right idea. CO2 does dissolve
in water to form a weak acid, so more
CO2 bubbled through water means lower
pH. But even all the CO2 in the
atmosphere only drops the pH of rain from 7 to 6.5
or so. (That's pristine rain, without the extra
acids from smog.) The change in the CO2
concentration in the air we breathe out is tiny.
You may not be able to measure the tiny drop in pH
you'll get with a pH meter. Then again, you may.
Quick and dirty: - Measure a change in
the person's respiration rate. This is the number
of breaths a person takes per minute. This will
give you some idea of the increase in the person's
respiration due to exercise, but of the change in
CO2 concentration in the air they
exhale. Typically, we inhale air with 20.84%
O2 and 0.04% CO2 and exhale
air with 13.6% O2 and 5.3%
CO2. I'm not an exercise physiologist,
but I'm guessing that during exercise, these
numbers probably don't change much. What does
change is the number of times we breathe in and
out and the amount of air we take in and expell
with each breath. This is how we rid our blood of
CO2 faster and get more O2
to our cells: we breathe faster and deeper, not
more efficiently. (Plus changes in heart rate and
volume.) (As a side note: marine mammals remove
much more O2 from the air than we do
with each breath: there is a greater difference
between the O2 concentration of air
going in the lungs and coming back out. This is
ONE thing that enables them to go longer between
breaths.)If you have a budget: - Add a pH
indicator to the water. Perhaps this is what you
suggested to your instructor already. The pH
indicator will change color as the pH of the water
changes color. Here's a description from a I found
on the web: "To compare your CO2 output
under different conditions, you will exhale
through a tube into a bottle partly filled with
the pH indicator solution. The CO2 that
you exhale will dissolve in the water, and
gradually acidify it. You'll be able to see the pH
indicator change color as this happens. By
measuring how long it takes for the pH change to
occur (and always exhaling at the same rate), you
will have a relative measure of the amount of
CO2 in your breath. The less time it
takes for the color change to happen, the more
CO2 there was in your breath."
science_b -
You can buy carbon dioxide sensors that measure
CO2 concentration in the air. I'm not
sure how expensive these are. Again, remember that
the concentration of CO2 in the breath
won't change, it will be the overall amount of
CO2 exhaled (lung volume x number
breaths).
project_sights -
The way excercise physiologists measure an
athlete's performance during exercise it is
open-circuit spirometry: they collect the air
athletes exhale while they are exercising at a
specific intensity over a specific time and then
"scrub" the CO2 out of the air and
measure it. You'd have to have a re-beather system
for this, which delivered clear air, recovered
expelled air, and then ran the old air through a
filter with soda lime. This is kind of similar to
the way re-breathers work for SCUBA divers. Click Here to return to the search form.
|
|
|
|
|
Copyright © 2020 The Regents of the University of California,
All Rights Reserved.
UCSB Terms of Use
|
|
|