Answer 1:
Your question is so specific, it sounds like a
science fair project! While I study
microorganisms, I don't study yeast. I'll will try
my best to answer your question, however.
First, a little background on fermentation:
Every organism on the planet needs a source of
energy for life and growth. Organisms that don't
photosynthesize get this energy by oxidizing
chemicals (e.g. carbon dioxide, glucose) with
the help of an added substance that accepts
electrons, known as an electron acceptor.
(Oxidation simply means a loss of electrons.) The
electron acceptor can be oxygen, iron, pyruvate,
sulfate, carbon dioxide or other compounds.
Most of the time, animals get their energy via
a complex metabolic process known as the citric
acid (or TCA) cycle, wherein oxygen is the
electron acceptor. This particular process
generates the most amount of energy per unit
organic matter oxidized. Many other processes,
which employ different electron acceptors, can
also generate energy. Bakers' yeast is part of
a group of microorganisms that gain energy through
fermentation. In fermentation, organic matter
serves as both the energy source and the electron
acceptor, and the energy yield is 18 times lower.
There are different fermentation pathways, and
fermentation products can include acids (such as
the lactic acid that builds up in your muscles) or
carbon dioxide plus ethanol (as with Saccharomyces
cerevisiae). The alcohol in beer, wine, whisky,
etc are all produced through fermentation, and
foods such as yogurt, cheese and most types of
bread depend upon fermentation as well. Yeasts are
very commercially important organisms.
To answer your question (finally!): The
relationship between oxygen availability and
ethanol production in yeast is complex. The
production of ethanol by Saccharomyces cerevisiae
is done commercially in large quantities, both for
biofuel and for alcoholic beverages, and so
manufacturers are interested in finding ways to
maximize ethanol production. For this reason, the
effect of oxygen on ethanol production has been
fairly well studied. The presence of oxygen at
normal atmospheric concentrations will inhibit any
fermentation process. At very low concentrations,
however, oxygen can actually increase the yield of
ethanol. This is sometimes referred to as the
microaerobic effect. As the level of oxygen
is increased beyond this point, byproducts such as
glycerol and acetic acid (vinegar) are produced by
the yeast in addition to ethanol, and the yield
and purity of the ethanol are reduced. As I
mentioned before, fermentation will stop
altogether once oxygen concentrations become too
high. This is sometimes referred to as the
Pasteur effect.
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Answer 2:
Both yeasts and people get energy for our life
processes and activities from breaking down
molecules like glucose. The first step is called
glycolysis (which, conveniently, means "sugar
breakdown"). The end product of this process is
called pyruvic acid. If there's oxygen around,
this product is broken down even further to carbon
dioxide and water to release a lot more energy.
This is called "aerobic respiration".
In
animals like us, when there's not enough oxygen
around we turn the pyruvic acid into lactic acid
until we have enough oxygen to get back to normal.
Lactic acid is one of the reasons your muscles get
sore when you exercise hard enough that you can't
keep up with your body's oxygen demand ("anerobic
respiration"). In yeasts, the pyruvic acid is
turned into ethanol and carbon dioxide when
there's a lack of oxygen. Ethanol is a poison,
though, so they will produce it until the solution
around them is about 12% alcohol, then they die
from ethanol poisoning. So yeasts do aerobic
respiration when they can because they get a lot
more energy from their food and don't produce the
poisonous ethanol. When they can't do aerobic
respiration, they switch to anearobic respiration
or fermentation. What gas do you think is in
the holes in bread?
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