Answer 1:
I’ve been looking into this, and it appears
that you will have to obtain several different
components to make the media for your
project.
Callus induction medium:
A fairly simple recipe I’ve found to make this
requires just three things, water, agar, and
Murishige and Skoog Basal Salts mixture. To
make 1 L of this mixture, add the ingredients in
the following amounts:
4.3 grams Murashige and Skoog
7.5 grams Agar
1 L of H2O
The links to purchase these items from sigma
aldrich are
click here (Murashige and Skoog,
product M 5524)
click here (Agar,
product 05040)
After mixing these ingredients together in a
large (at least 2 L) Erlenmeyer flask (erlenmeyer flask), you are supposed to
autoclave them. An autoclave is a machine that
uses steam and pressure to sterilize media and
tools. The autoclave step is necessary not only
for sterilization but also to dissolve the
powdered agar in the water. However, if you do
not have access to an autoclave you can still
dissolve the agar in the liquid by bringing it
to a boil on a hot plate, but this method will
not sterilize your media. If you prefer this
method, I would use a beaker instead of an
Erlenmeyer flask (although either works). After
you have dissolved the agar, wait until the
flask/beaker is cool enough to touch (but not so
cool that the agar has solidified again) and
pour ~25 mL into a petri plate
(
labware,
polystyrene 100mm x 15mm, P5856, would work
best). Repeat this until you have used up all
the medium (should make ~40 petri plates).
Clone Inducing Medium:
Unfortunately I was having some trouble finding
a recipe for this one. However, in my searching
I did come across this kit: science
education
This kit includes the clone inducing medium
and the callus inducing medium, but it is a wee
bit pricey (over $130). I will keep searching
for a recipe but hopefully this will help you
for now! Email me if you have any questions
about how to make media (I make lots of media in
lab so I should be able to help you with any
trouble you may have).
Good luck!
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