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I was wondering if you know where can I buy callus initiation medium and clone induction medium? I'm doing a project dealing with plant cloning. If you know where to get them that would be a big help!
Question Date: 2012-10-16
Answer 1:

I’ve been looking into this, and it appears that you will have to obtain several different components to make the media for your project.

Callus induction medium:
A fairly simple recipe I’ve found to make this requires just three things, water, agar, and Murishige and Skoog Basal Salts mixture. To make 1 L of this mixture, add the ingredients in the following amounts:

4.3 grams Murashige and Skoog
7.5 grams Agar
1 L of H2O

The links to purchase these items from sigma aldrich are

click here (Murashige and Skoog, product M 5524)

click here (Agar, product 05040)

After mixing these ingredients together in a large (at least 2 L) Erlenmeyer flask (erlenmeyer flask), you are supposed to autoclave them. An autoclave is a machine that uses steam and pressure to sterilize media and tools. The autoclave step is necessary not only for sterilization but also to dissolve the powdered agar in the water. However, if you do not have access to an autoclave you can still dissolve the agar in the liquid by bringing it to a boil on a hot plate, but this method will not sterilize your media. If you prefer this method, I would use a beaker instead of an Erlenmeyer flask (although either works). After you have dissolved the agar, wait until the flask/beaker is cool enough to touch (but not so cool that the agar has solidified again) and pour ~25 mL into a petri plate

( labware, polystyrene 100mm x 15mm, P5856, would work best). Repeat this until you have used up all the medium (should make ~40 petri plates).

Clone Inducing Medium:
Unfortunately I was having some trouble finding a recipe for this one. However, in my searching I did come across this kit:

science education

This kit includes the clone inducing medium and the callus inducing medium, but it is a wee bit pricey (over $130). I will keep searching for a recipe but hopefully this will help you for now! Email me if you have any questions about how to make media (I make lots of media in lab so I should be able to help you with any trouble you may have).

Good luck!

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