Hi there,
Recently in class I have done a Redox titration
to determine the concentration of ascorbic acid
within some orange juice. The pH of the solution
at the beginning and the end were basically the
same and I was wondering why? From what I have
learned of titrations curves, the pH should have
changed, at least a bit... I used the Potassium
Iodate method if that is of any help. Thank you
for your help!
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Answer 1:
There are always incredibly frustrating ways
experiments can go wrong, even at a professional
level. Were the two solutions well-mixed? If you
are mixing two solutions that start at different
pHs, the resulting solution will always be
somewhere between the two initial pHs. If you are
seeing no change, it is possible one of the
solutions (either KIO3 or orange juice)
was not at the initial pH you suspected. If the
pHs are too close, there won't be much change in
the final solution. If the pHs are significantly
different but you see no change, perhaps you did
not add enough of the base to raise the orange
juice pH. This would be fixed just by adding more
KIO3.
As always (and especially in high school labs)
the chemicals could be very impure or
contaminated, so that could cause issues.
If you add significant amounts of
KIO3, the pHs are very different before
mixing, and you STILL see no change, the solution
could be what's called a "buffer." (If you already
know what a buffer is, feel free to skip the rest
of this answer.) Buffers are solutions that are
relatively stable in pH despite addition of acid
or base. This is done by weak acids or bases
exchanging free hydrogens with the strong acid or
base, preventing too much change in the ion
content of the solution. Most chem textbooks
discuss this more thoroughly, and this link
is a good resource. Many biological fluids (the
textbook example is blood) are buffers because if
you didn't have molecules stabilizing pH, the
fluids would be very sensitive pH changes in the
environment and would likely kill the organism. I
don't know enough to say for absolute certain that
this is a buffer reaction, but it may be. |
Answer 2:
Ascorbic acid is a fairly weak acid, and
potassium iodate may be chemically active itself.
Maybe the concentration of ascorbic acid was so
large that you couldn't titrate it all, or that
there were too many other things (e.g. citric
acid) in the orange juice for it to matter.
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