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How can I isolate chloroplasts from spinach leaves and make a solution viable for it to be used in a spectrometer, keeping in mind the chloroplasts still need to be able to photosynthesize? What would be a good phosphate buffer to use with this chloroplast solution, DPIP, and distilled water?
Question Date: 2018-05-17
Answer 1:

You need to macerate the spinach and break up the cell walls, but keep the buffer solution right. Distilled water is obviously wrong, but I'm not sure DPIP would work either (if DPIP is what I think it is). You want the same concentrations of solutes that are present in a spinach cell.



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