How can I isolate chloroplasts from spinach
leaves and make a solution viable for it to be
used in a spectrometer, keeping in mind the
chloroplasts still need to be able to
photosynthesize? What would be a good phosphate
buffer to use with this chloroplast solution,
DPIP, and distilled water?
Question Date: 2018-05-17
You need to macerate the spinach and break up the
cell walls, but keep the buffer solution right.
Distilled water is obviously wrong, but I'm not
sure DPIP would work either (if DPIP is what I
think it is). You want the same concentrations of
solutes that are present in a spinach cell.