Answer 1:
Ultraviolet light kills cells (including
bacteria) by damaging their DNA beyond repair.
UV, especially UVC, is in the wavelength range
that is shorter than visible light and can
penetrate into cells. Cells are generally
pretty good at recognizing and repairing DNA
damage. However, if repair is not achieved, this
results in mutation, which can be deleterious,
such as in the case of mutations that occur in
genes that drive the onset of cancer - think UV
exposure (in the UVA and UVB range) via sunlight.
Under extreme circumstances, as in the case of
UVC exposure, the DNA damage is much more
extensive and often cannot be repaired. Thus,
the cell's set of "instructions" for how to
respond to stimuli, how to carry out metabolism,
and generally function is compromised, leading
to death.
In 1878, Downes and Blunt published a paper
describing how shortwave light could kill
("sterilize") bacteria, so we have known about
this for a while. A highly cited paper from 2012
described how using UV light to treat infected
wounds in mice was effective - at least as
good as if not better than standard antibiotic
treatment:
T. Dai, B. Garcia, C.K. Murray, M.S. Vrahas, and
M.R. Hamblin, 2012. UVC light prophylaxis for
cutaneous wound infections in mice. Antim. Agents
Chemother. 56:3841-3848.
This paper, coupled with the rise in antibiotic
resistance, led to an increase in investigating
the clinical use of UV in treating bacterial
infections. Clinical use is tricky -
wavelength, dose time and focused application
are critical. Inappropriate use could lead to
cancer in the patient's normal cells! (Take home
message - avoid getting a sunburn!)
What is the nature (mechanism) of the damage
that causes death? I'm guessing this might be
the deeper context of your question - how can
light damage DNA and thus kill a bacterial (or
any) cell? This question is a great example
of why we need to understand chemistry in order
to fully understand biology.
UVC triggers a chemical reaction in the
pyrimidine base pairs in DNA. UVC causes a
photochemical reaction in the DNA. It
disrupts the bonding between the
complementary pairs and when the light is absorbed
by thymines (one of the bases) that happen to sit
adjacent to each other, it can result in a
chemical reaction that causes covalent bonding to
create a thymine dimer. Widespread disruption
of the complementary base pairing and large
numbers of unrepaired dimers can lead to
catastrophic consequences for the cell.
|
Answer 2:
Ultraviolet light is powerful enough to break
chemical bonds. This includes the bonds that
make up DNA. By irradiating bacteria with
high-energy UV, you break up the DNA and
thereby destroy their ability to make
proteins, which are created using the
information in DNA (the UV will break up the
proteins themselves, too). Since bacteria live by
making and using proteins in chemical reactions,
destroying the DNA they use to make proteins and
the proteins themselves that they use kills the
bacteria.
Click Here to return to the search form.
|