UCSB Science Line
Sponge Spicules Nerve Cells Galaxy Abalone Shell Nickel Succinate X-ray Lens Lupine
UCSB Science Line
Home
How it Works
Ask a Question
Search Topics
Webcasts
Our Scientists
Science Links
Contact Information
I know that transgenic e.coli has been used to produce human insulin - but is this done in vitro and then the bacteria are separated from the product which will later be injected by the diabetic? Or are the transgenic e.coli somehow transplanted back into a diabetic and then do their insulin production inside of the body for immediate use?
Answer 1:

Large quantities of essentially human insulin is made by bacterial cells in vitro. The Insulin messenger RNA (mRNA) is first extracted from human pancreatic cells, then complementary DNA (cDNA) is made using the enzyme reverse transcriptase. Along with a bacterial regulator gene, the cDNA is incorporated (ligated) into the bacterial plasmid. The transformed plasmid vector is then reinserted into a bacterial cell where it replicates. All the plasmids code for the production of proteins, including the human insulin. Large quantities can be made this way for use by diabetics.The insulin is the extracted for human use.


Click Here to return to the search form.

University of California, Santa Barbara Materials Research Laboratory National Science Foundation
This program is co-sponsored by the National Science Foundation and UCSB School-University Partnerships
Copyright © 2015 The Regents of the University of California,
All Rights Reserved.
UCSB Terms of Use